IJERT-EMS
IJERT-EMS

Rapid Identification of Human Pathogenic Vibrio Species in Fresh Water using Multiplex PCR


Rapid Identification of Human Pathogenic Vibrio Species in Fresh Water using Multiplex PCR
Authors : Amit Ranjan Prasad Singh, Manisha, Manvinder Singh
Publication Date: 29-07-2015

Authors

Author(s):  Amit Ranjan Prasad Singh, Manisha, Manvinder Singh

Published in:   International Journal of Engineering Research & Technology

License:  This work is licensed under a Creative Commons Attribution 4.0 International License.

Website: www.ijert.org

Volume/Issue:   Volume. 4 - Issue. 07 , July - 2015

e-ISSN:   2278-0181

 DOI:  http://dx.doi.org/10.17577/IJERTV4IS070139

Abstract

In the present investigation we focus on identification of Vibrio species in different rivers and ponds of Indian northern plains. This study targets five species of Vibrio such as Vibrio alginolyticus, Vibrio cholerae, Vibrio mimicus, Vibrio parahaemolyticus, Vibrio vulnificus which are the major human disease causing Vibrio species. Primarily identification of Vibrio species done through gram’s staining and further by color of colonies developed on TCBS agar plates. A multiplex PCR assay had been developed for the detection of targeted five species of Vibrio for the DNA isolated from various samples of water using specific primers targeting tox gene. The assay was specific as no amplification occurred for other bacterial DNA.

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