Author(s): Amit Ranjan Prasad Singh, Manisha, Manvinder Singh
Published in: International Journal of Engineering Research & Technology
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume/Issue: Volume. 4 - Issue. 07 , July - 2015
In the present investigation we focus on identification of Vibrio species in different rivers and ponds of Indian northern plains. This study targets five species of Vibrio such as Vibrio alginolyticus, Vibrio cholerae, Vibrio mimicus, Vibrio parahaemolyticus, Vibrio vulnificus which are the major human disease causing Vibrio species. Primarily identification of Vibrio species done through gramâ€™s staining and further by color of colonies developed on TCBS agar plates. A multiplex PCR assay had been developed for the detection of targeted five species of Vibrio for the DNA isolated from various samples of water using specific primers targeting tox gene. The assay was specific as no amplification occurred for other bacterial DNA.
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