Author(s): Abeer S. Emam, Yehia A. Badr
Published in: International Journal of Engineering Research & Technology
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume/Issue: Volume. 5 - Issue. 04 , April - 2016
Biomedical optics is a fast growing field of research. The development of a technique that is less invasive and provides real time and quantitative information about tissue biochemistry is of great importance for cancer diagnosis. Microscopy enables the scientist to see and elucidate special features and capabilities that allow different cell types to thrive in different environments and to perform different functions. One of the greatest advances in light microscopy within the past two decades is the development of confocal laser scanning microscope system (CLSMS ) which is a relatively new light microscopical imaging technique, and is considered a very successful tool that has greatly impacted biological research and became a valuable tool in many fields of study, due to its superior axial and lateral resolution over wide-field imaging but it is a very expensive tool. The work presented in this thesis is intended to serve as a primer for basic confocal microscopy theory as well as a description of a low-cost method of design and construction of a home-made confocal laser scanning microscopy system. We have provided a simple and affordable method for building a confocal scanning laser microscopy system on a modest budget with decent lateral resolution and 2D dissection capability comparable to that of commercial systems. The digital images obtained as the results of our system (AY128R) CLSMS are compared to the digital images from the commercial CLSMS ,which is about 80% similar to original image the structure of the neoplasia cells and malignant cells are appear. Physical and optical comparative study between two optical techniques ( microscopic and spectroscopic ) techniques with previous study for FT-Raman and FTIR spectroscopic techniques on IDC of breast samples was illustrated to determine the characterized features, advantage, similarities and differences for each optical techniques for early diagnosis breast cancer tissues.Both techniques are widely explored for analysis of tissue and cells and ultimately biomedical applications. Comparison of the spectroscopic and microscopic techniques demonstrates clear differences between the performances of two methods. Two techniques are complementary with each other. The combination of an optical microscopy and spectroscopy leads to the development of a new and powerful analytical characterization technology.
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