Author(s): Dr. Indrani Jadhav, Dr. Kapilesh Jadhav, Roshan Vasniwal, Dr. Divya Shrivastava
Published in: International Journal of Engineering Research & Technology
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume/Issue: Volume. 5 - Issue. 09 , September - 2016
The Gram positive, spore forming bacterium was isolated from textile effluent contaminated soil and was examined for its ability to degrade azo dye (deep red glx). On analysis of 16S rRNA gene sequence, strain showed 99% sequence similarity with Bacillus cereus ATCC 14579 and belongs to group Firmicutes of domain Bacteria. The strain has been assigned name BPL and the gene sequence was deposited in Genbank with accession number KU510086. The Bacillus cereusstrain BPL was able to decolorize deep red glx with efficiency of 78% in minimal salt medium (MSM) supplemented with dye at concentration of 0.5gl-1. Decolorization studies have shown that the strain was able to decolorize deep red glx in pH range of 4-9 at 37oC with optimum pH of 9 and adaptability to decolorize dye in alkaline conditions. Other parameters such as nitrogen sources, carbon sources and osmotic concentration were also examined. When supplemented with nitrogen source (peptone, 5gl-1), carbon source (maltose, 5gl-1) and NaCl (10gl-1), the decolorization obtained were 85%, 87% and 82% respectively. The Bacillus cereus strainBPL was found to decolorize azo dye deep red glx and shows good possibilities for biodegradation and decolorization of textile effluents.
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