GC-MS Analysis of Pentatropis Microphylla

GC-MS Analysis of Pentatropis Microphylla

1*Kalaivanan C, 1Assistant Professor, Department of Chemistry,

K. Ramakrishnan College of Technology, Tiruchirappalli

3Kokila S,

3Assistant Professor, Department of Microbiology,

Chennai Medical College Hospital & Research Centre (SRM Group),

Tiruchirapplli

2Sundhararajan A, 2Assistant Professor, Department of Biochemistry,

Chennai Medical College Hospital & Research Centre (SRM Group),

Tiruchirapplli

4Ahmed John S.

4Professor,

PG & Research Department of Botany, Jamal Mohamed College (Autonomus), Tiruchirappalli

Abstract Background

The present study was aimed to determine the presence of biomolecules in the ethanolic leaves extract of Pentatropic microphylla

Materials and Methods

Pentatropic microphylla leaves extract was used. Gas Chromatography Mass Spectrum equipment used for identification and determine the bio active compounds.

Results

The GC-MS results have shown the different type of secondary metabolites. A total of 28 bio active compounds were identified by GR-MS. Out of 28 compounds 2 compounds highly potent 1,6-Anhydro-á-D-glucopyranose (28.7332%) and Propane, 1,1,3-triethoxy (25.9192%) respectively.

Conclusion

From the results, it is evident that Pentatropic microphylla contains various phytocomponents and is recommended for further evaluation of its anti diabetic or anticancer activity.

Key words: Pentatropic microphylla, 1,6-Anhydro-á-D- glucopyranose, Propane, 1,1,3-triethoxy

INTRODUCTION

Thousands of secondary plant products have been identified and it is estimated that thousand of these compounds still exist. Since secondary metabolites from natural resources have been elaborated with in living system, they are often perceived as showing more drug-likeness and biological friendliness than totally synthetic molecules making those good candidates for further drug development (Koehn 2005).

Pentatropis microphylla belongs to the family Asclepiadaceae twining, perennial herb. Leaves are 1-3.5 cm long, 0.5-2.5 cm wide broadly oblong or ovate elliptic.

Plant material Pentatropis microphylla leaves was collected in Tiruchirappalli District of Tamil Nadu.

PLANT SAMPLE EXTRACTION

The samples were cleansed through tap water and air dried at room temperature for 3 weeks and grounded into powder. The 20gm dried powder material was extracted successively using ethanol solvent to soxhlet extractor. The

crude extract was stored at 40 C until use. Take 2 mg of crude extract to dissolve 5ml of absolute alcohol for few Minutes and then filtered through Whatman filter paper No.1, before filtering, the filter paper along with sodium sulphate is wetted with alcohol. The filtrates are then concentrated by bubbling nitrogen gas into the solution and reduce the volume to 1ml. The extract contains both polar and non-polar phytoconstituents.

GC MS ANALYSIS

GC-MS analysis was carried out on a GC Clarus

500 Perkin Elmer system comprising a AOC-20i auto sampler and gas chromatograph interfaced to a mass spectrometer (GC-MS) instrument employing the following conditions. Column Elite-5ms fusedsilica capillary column (30mmx0.25mm ID x 1µ M df, composed 5% Phenyl 95% dimethylpolysiloxane), operating in electron impact mode at

70 eV helium (99.999%) was used as carrier gas at a constant flow of 1ml/min and an injection volume of 0.5 µ 1was employed (split ratio of 10:1) injector temperature 2500C, ion source temperature 280 0C. The oven temperature was programmed from 110 0C (isothermal for 2min), with an increase of 100C/min, to 2000C, then 50C/min to 2800C, ending with a 9min isothermal at 2800C. Mass spectra were taken at 70 eV, a scan interval of 0.5 seconds and fragments from 45 to 450 Da. Total GC running time was 36 minutes.

IDENTIFICATION OF ACTIVE COMPOUNDS

Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) having more than 62,000 patterns. The spectrum of the unknown compound was compared with the spectrum of the known compound stored in the NIST library. The name, molecular weight and structure of the compounds of the test material were ascertained.

RESULTS AND DISCUSSION

The results were shown in Figure 1. Totally twenty eight compounds were identified in the ethanolic leaves extract of Pentatropis microphylla by GC-MS analysis. The

active principles were compared with standard library and identified. The active principles retention time (RT), molecular formula, molecular weight (MW) and concentration (%) are presented in the Table 1. Among the twenty eight compounds two compounds were identified as most prevailing compounds such us 1,6-Anhydro-á-D- glucopyranose (28.7332%) and Propane, 1,1,3-triethoxy- (25.9192%).

As many researchers reported that most of the biomolecules have anti microbial or anti diabetic activity. Since, there is no evidence or previous study conducted in this plant. Hence, the authors recommended further research to identify its anti diabetic or anti cancer activity.

3-Hexanol, 2,2-dimethyl

Figure 1: Pentatropis microphylla Chromatogram