Biochemical Assay of Eryngium Foetidum and Ricinus Communis

Biochemical Assay of Eryngium Foetidum and Ricinus Communis

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Rukaiya Khatoon*, Abhipsita De*, Sherry Rajan* and Malavika Bhattacharya#

Department of Biotechnology Techno India University, West Bengal

Kolkata, India

Abstract The manuscript is about two medicinal plants that have been in use since ages in the traditional regions. The objective of this work is to enlighten the biochemical properties of these two plants by analyzing the samples of their leaves in qualitative as well as quantitative ways. The name of the chosen medicinal plant samples are Eryngium Foetidum and Ricinus Communis. Eryngium foetidum is commonly known as Ban dhaniya and Ricinus Communis as Castor plant in India. The introduction of the project mentions a detailed review of both plants and their specified benefits that have been observed in the past research. Both the species are abundantly found in the hilly regions of India. The materials and methods portion specifies the procedure of experiments carried put for the purpose of analyzing the biochemical properties of the plant sample. The qualitative analysis of the plants showed the test results for benedicts test, ethanol test and the iodine test. Benedicts test determines the sugar content in the sample, ethanol test is for lipid analysis and the iodine test checks for the presence of starch in the plant leaves. As the leaves were dried before the test was done, the results for qualitative analysis came negative. On the other hand, there was conducted a test regarding pH identification, amino acid content and the polysaccharide content determination. The last two tests had been measured by determining the absorbance of the sample in the UV- spectrophotometer.

KeywordsBiochemical attributes; Plant extracts; Dietary habits; Medicinal plants

I. INTRODUCTION

Medicinal plants are regarded as a source for curing a number of diseases. This project is about the use and characteristics of two such medicinal plants that have many benefits with regards to curing a number of problems that otherwise require a lot of money in their allopathic treatment (Sisodiya and Shrivastava, 2017; Prabhavathi et al., 2016). There are certain biochemical tests done for both the samples. Qualitative and quantitative analysis of Eryngium foetidum and Ricinus communis were carried out in this work.

II. MATERIALS AND METHODS

Preparation of the extractsProcedureB. Ethanol test2. Quantitative analysisA. pH testB. Amino Acid TestC. Polysaccharide testA. Qualitative analysisB. Quantitative analysis1. pH test1. Benedicts test2. Ethanol test3. Iodine test2. Polysaccharide testGraph for absorbance at 488nmQuantitative analysis1. Amino acid test

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